Public Health Laboratory Action For Elucidation of Diarrhea Outbreak by Cyclospora cayetanensis in General Salgado Municipality , SP
Keywords:
Cyclospora cayetanensis, diarrhea outbreak, parasite
Abstract
The aim of this study was to report the occurrence of diarrhea outbreak by Cyclospora cayetanensis inGeneral Salgado Municipality, SP, and the role of the Public Health Laboratory for its elucidation, andposterior monitoring of patients with diarrhea. At Regional Laboratory of Adolfo Lutz Institute in São Josédo Rio Preto - SP, during the period from August 1999 to December 2002, analyses of samples that camefrom General Salgado Municipality, SP were carried out. The following assays were performed: 103 waterpotability analyses, according to American Public Health Association guidelines; 130 coprocultures,including nine examinations for cholera, according to the technique described by Pessoa et al, 1983 andCholera’s National Commission Manual, 1992; 132 faeces parasitological examinations, includingopportunistic agents (by means of autofluorescence assay, alcohol - acid resistant staining by modifiedKinyoun technique, formol - ether sedimentation, sporulation by 2.5% potassium dichromate, and Hoffman technique), and 85 virus detection by means of EIARA (m) - Bio Manguinhos/FIOCRUZ, Rio de Janeiro -RJ. Of coproculture examinations, nine (6.9%) were positive for: Shigella boydii, Shigella sonnei,Escherichia coli, O11 and O128, Salmonella Typhimurium, Salmonella Saintpaul, Salmonella Enteritidis,Salmonella Oranienburg, and Salmonella spp (non serotyped). From water samples analyses, 23 (22.3%)were positive for bacterias from coliform group, and 4 four samples (17.4%) were also positive for fecalcoliforms. Rotavirus antigen was detected in two (2.4%) fecal samples; and the occurrence of Cyclosporacayetanensis was observed in 16 (12.1%) samples. Owing to the investigation delineated for solving theinjury threatening the health of population from General Salgado Municipality, the first outbreak ofCyclospora cayetanensis in Brazil could be described.References
1. American Public Health Association. Standard Methods for the
examination for water and wastewater, 18 th ed., 1992.
2. Ash, L. R.; Orihel, T. C. Atlas of Human Parasitology.
Chicago:ASCP PRESS; 1997. 410p.
3. Ashford, R. W. Occurrence of na undescribed coccidian in Papua New
Guinea. Ann Trop. Med. Parasitol, v. 73, p. 497-500, 1979.
4. Berlim, O. G. W. et al. Recovery of Cyclospora organisms from patients
with prolonged diarrhea. Clin. Infect. Dis., v. 18, p. 606-9, 1994.
5. Bern, C. et al. Epidemiologic studies of Cyclospora cayetanensis in
Guatemala. Emerging Infectious Diseases, v. 5, n. 6, 1999.
6. Centers of Diseases Control and Prevention - CDC. Uptade: outbreaks
of Cyclospora cayetanensis infection - United States and Canada,
MMWR, v. 45, p. 611-2, 1996.
7. Centro de Vigilância Epidemiológica - Secretaria de Estado da Saúde -
SP. Boletim Informativo, ano 17, n. 60, 2002.
8. Centro de Vigilância Epidemiológica - Secretaria de Estado da Saúde -
SP. Ciclosporíase / Cyclospora cayetanensis. Manual de doenças
transmitidas por alimentos - INFORME-NET DTA [http:/
www.cve.saude.sp.br/htm/hidrica/inf_cyclospora.htm].17 fevereiro
2001.
9. Centro de Vigilância Epidemiológica - Secretaria de Estado da Saúde -
SP. Surto de diarréia relacionada com alimentos: um estudo caso -
controle, General Salgado - SP, 1999-2000. [ftp.cve.saude.sp.gov.br/
doc_tec/hidrica/dta_inqsurtogs.ppt]. 26 janeiro 2004].
10. De Carli, G. A. Parasitologia clínica: diagnóstico de laboratório
dos coccídios e microsporídios intestinais. Edipucrs; 2000. 73p.
11. Eberhard, M. L. et al. Cyclospora cayetanensis Infections in Haiti: a
common occurrence in the absence of watery diarrhea. Am. J. Trop.
Med. Hyg., v.60, n. 4, p. 584-6, 1999.
12. Eberhard, M. L.; Pieniazek, N. J.; Arrowood, M. J. Laboratory diagnosis
of Cyclospora infections. Arch .Pathol.Lab.Med., v. 121, 1997.
13. Germano, M. P. L.; Germano, M. I. S. Higiene e Vigilância
Sanitária de Alimentos. São Paulo: Livraria Varela; 2001. 629p.
14. Hoge, C. W. et al. Prevalence of Cyclospora species and other enteric
pathogens among children less than 5 years of age in Nepal. J. Clin.
Microbiol., v. 33, p. 3058-60, 1995.
15. Hoge, C. W. et al. Epidemiology of diarrhoeal illness associated with
coccidian-like organism among travellers and foreign residents in
Nepal. Lancet, v. 341, p. 1175-9, 1993.
16. Instituto Adolfo Lutz - São Paulo, SP - 100 anos do Laboratório de
Saúde Pública. São Paulo: Letras & Letras; 1992. 280p.
17. Leventhal, R.; Cheadle, R. Parasitologia médica. 4ª ed. Editorial
Premier Ltda; 1997. p. 124-6.
18. Lima, A. O. et al. Métodos de laboratório aplicados à clínica. 5ª
ed. Guanabara Koogan; 1977. 669p.
19. Madico, G. et al. Epidemiology and treatement of Cyclospora
cayetanensis infection in Peruvian children. Clin.Infect.Dis., v. 24,
p. 977-81, 1997.
20. Ministério da Saúde. Comissão Nacional de Prevenção da Cólera -
CNPC - Manual de Diagnóstico Laboratorial da Cólera. Brasília:
1º ed.; 1992.
21. Neves, D. P. et al. Parasitologia Humana. São Paulo: Atheneu;
2000. 428p.
22. Ortega, Y. R. et al. Isolation of Criptosporidium parvum and Cyclospora
cayetanensis from vegetables collected in markets of na endemic
region in Peru. Am. J. Trop. Med. Hyg., v. 57, n. 6, p. 683-6, 1997.
23. Ortega, Y. R. et al. Cyclospora species: a new protozoan pathogen of
humans. N. Engl. J. Med., v.328, p. 1308-12, 1993.
24. Ortega, Y.R.; Sterling, C.R.;Gilman,R.H. Cyclospora cayetanensis.
Advanc. Parasit., v. 40, p.399-418, 1998.
25. Pessoa, G. V. A et al. Ocorrência de enterobactérias em São Paulo, no
septênio 1970-1976. I - Sorotipos de Salmonella isolados e
identificados. Rev. Inst. Adolfo Lutz, n. 38, p. 97-103, 1983.
26. Pessoa, S. B.; Martins, A.V. Parasitologia médica. 10 ed. Guanabara
Koogan; 1977. p. 124-6.
27. São Paulo, Secretaria da Saúde do Estado de São Paulo, Coordenadoria
dos Institutos de Pesquisa da Secretaria de Saúde. Atas do Fórum
Saúde. São Paulo; 1992. 302p.
28. Soave, R.; Herwaldt, B. L.; Relman, D. A. Cyclospora. In: Hughes, J.
M.; Conte, J. E. editors. Infectious Disease Clinics of North
America, v. 12, n. 1, Philadelphia: W. B. Saunders Company, 1998.
29. Tauxe, R. V. Emerging foodborne diseases: an evoluing public health
challenge. Emerging Infectious Diseases, v. 3, n. 4, p. 425-34, 1997.
examination for water and wastewater, 18 th ed., 1992.
2. Ash, L. R.; Orihel, T. C. Atlas of Human Parasitology.
Chicago:ASCP PRESS; 1997. 410p.
3. Ashford, R. W. Occurrence of na undescribed coccidian in Papua New
Guinea. Ann Trop. Med. Parasitol, v. 73, p. 497-500, 1979.
4. Berlim, O. G. W. et al. Recovery of Cyclospora organisms from patients
with prolonged diarrhea. Clin. Infect. Dis., v. 18, p. 606-9, 1994.
5. Bern, C. et al. Epidemiologic studies of Cyclospora cayetanensis in
Guatemala. Emerging Infectious Diseases, v. 5, n. 6, 1999.
6. Centers of Diseases Control and Prevention - CDC. Uptade: outbreaks
of Cyclospora cayetanensis infection - United States and Canada,
MMWR, v. 45, p. 611-2, 1996.
7. Centro de Vigilância Epidemiológica - Secretaria de Estado da Saúde -
SP. Boletim Informativo, ano 17, n. 60, 2002.
8. Centro de Vigilância Epidemiológica - Secretaria de Estado da Saúde -
SP. Ciclosporíase / Cyclospora cayetanensis. Manual de doenças
transmitidas por alimentos - INFORME-NET DTA [http:/
www.cve.saude.sp.br/htm/hidrica/inf_cyclospora.htm].17 fevereiro
2001.
9. Centro de Vigilância Epidemiológica - Secretaria de Estado da Saúde -
SP. Surto de diarréia relacionada com alimentos: um estudo caso -
controle, General Salgado - SP, 1999-2000. [ftp.cve.saude.sp.gov.br/
doc_tec/hidrica/dta_inqsurtogs.ppt]. 26 janeiro 2004].
10. De Carli, G. A. Parasitologia clínica: diagnóstico de laboratório
dos coccídios e microsporídios intestinais. Edipucrs; 2000. 73p.
11. Eberhard, M. L. et al. Cyclospora cayetanensis Infections in Haiti: a
common occurrence in the absence of watery diarrhea. Am. J. Trop.
Med. Hyg., v.60, n. 4, p. 584-6, 1999.
12. Eberhard, M. L.; Pieniazek, N. J.; Arrowood, M. J. Laboratory diagnosis
of Cyclospora infections. Arch .Pathol.Lab.Med., v. 121, 1997.
13. Germano, M. P. L.; Germano, M. I. S. Higiene e Vigilância
Sanitária de Alimentos. São Paulo: Livraria Varela; 2001. 629p.
14. Hoge, C. W. et al. Prevalence of Cyclospora species and other enteric
pathogens among children less than 5 years of age in Nepal. J. Clin.
Microbiol., v. 33, p. 3058-60, 1995.
15. Hoge, C. W. et al. Epidemiology of diarrhoeal illness associated with
coccidian-like organism among travellers and foreign residents in
Nepal. Lancet, v. 341, p. 1175-9, 1993.
16. Instituto Adolfo Lutz - São Paulo, SP - 100 anos do Laboratório de
Saúde Pública. São Paulo: Letras & Letras; 1992. 280p.
17. Leventhal, R.; Cheadle, R. Parasitologia médica. 4ª ed. Editorial
Premier Ltda; 1997. p. 124-6.
18. Lima, A. O. et al. Métodos de laboratório aplicados à clínica. 5ª
ed. Guanabara Koogan; 1977. 669p.
19. Madico, G. et al. Epidemiology and treatement of Cyclospora
cayetanensis infection in Peruvian children. Clin.Infect.Dis., v. 24,
p. 977-81, 1997.
20. Ministério da Saúde. Comissão Nacional de Prevenção da Cólera -
CNPC - Manual de Diagnóstico Laboratorial da Cólera. Brasília:
1º ed.; 1992.
21. Neves, D. P. et al. Parasitologia Humana. São Paulo: Atheneu;
2000. 428p.
22. Ortega, Y. R. et al. Isolation of Criptosporidium parvum and Cyclospora
cayetanensis from vegetables collected in markets of na endemic
region in Peru. Am. J. Trop. Med. Hyg., v. 57, n. 6, p. 683-6, 1997.
23. Ortega, Y. R. et al. Cyclospora species: a new protozoan pathogen of
humans. N. Engl. J. Med., v.328, p. 1308-12, 1993.
24. Ortega, Y.R.; Sterling, C.R.;Gilman,R.H. Cyclospora cayetanensis.
Advanc. Parasit., v. 40, p.399-418, 1998.
25. Pessoa, G. V. A et al. Ocorrência de enterobactérias em São Paulo, no
septênio 1970-1976. I - Sorotipos de Salmonella isolados e
identificados. Rev. Inst. Adolfo Lutz, n. 38, p. 97-103, 1983.
26. Pessoa, S. B.; Martins, A.V. Parasitologia médica. 10 ed. Guanabara
Koogan; 1977. p. 124-6.
27. São Paulo, Secretaria da Saúde do Estado de São Paulo, Coordenadoria
dos Institutos de Pesquisa da Secretaria de Saúde. Atas do Fórum
Saúde. São Paulo; 1992. 302p.
28. Soave, R.; Herwaldt, B. L.; Relman, D. A. Cyclospora. In: Hughes, J.
M.; Conte, J. E. editors. Infectious Disease Clinics of North
America, v. 12, n. 1, Philadelphia: W. B. Saunders Company, 1998.
29. Tauxe, R. V. Emerging foodborne diseases: an evoluing public health
challenge. Emerging Infectious Diseases, v. 3, n. 4, p. 425-34, 1997.
Published
2004-12-30
How to Cite
ZINI, R. M., SANTOS, C. C. M., ALMEIDA, I. A. Z. C., PERESI, J. T. M., & MARQUES, C. C. (2004). Public Health Laboratory Action For Elucidation of Diarrhea Outbreak by Cyclospora cayetanensis in General Salgado Municipality , SP. Revista Do Instituto Adolfo Lutz, 63(1), 116-121. https://doi.org/10.18241/rial.v63i1.34819
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