Validation of acetate QuEChERS methodology for pesticides multiresidue analysis in soybean samples and soybean-soluble extract by means of high-performance liquid chromatography coupled to tandem mass spectrometry

Abstract

The possibility of occurring the soy and soy derivatives samples contamination with pesticide residues has become significant due to the increased use of these products in human nourishment. In this context, the present study aimed at validating the acetate QuEChERS multiresidue methodology for analyzing 144 pesticide residues in soybean samples and soybean soluble extract using high-performance liquid chromatography coupled to tandem mass spectrometry. The parameters evaluated were: selectivity (matrix effect in the two sample types), linearity (working range, regression significance, homogeneity of the regression residues), accuracy (recovery), precision (repeatability) and limits of detection and quantification. The analytical curves presented R2 ≥ 0.95 and r ≥ 0.98 in the working range (0.002 to 0.200 μg.mL-1). The accuracy and precision evaluated at two levels of fortification of two matrices presented values of recovery in the 70-120 % range and CV (%) ≤ 20 %, respectively. The limits of detection nd quantification showed satisfactory results. The validated methodology allowed to perform the determination of 122 substances in the matrix soybean and 124 in the soluble extract of soybean.