Ultrasonic waves in the preparation of an antigen for the diagnosis of chagas' disease

  • Marcelo O. A. Corrêa Instituto Adolfo Lutz, São Paulo, SP
  • Manoel de Brito e Silva Instituto Adolfo Lutz, São Paulo, SP
  • Vicente Amato Neto Universidade de São Paulo, Faculdade de Medicina, São Paulo, SP

Abstract

A new technic in the preparation of an antigen for the diagnosis of Chagas' disease by the complement fixation test (Machado-Guerreiro test) is presented. A suspension of trypanosomas in saline solution with 1/10,000 merthiolate was treated by ultrasonic waves in two 10-minute periods, using a frequency of 800 kilocycles in the first period and 2,400 kilocycles in the second one, with energy consumption of 30 and 38 watts, respectively. Preliminary results obtained in 87 sera suggest that the preparation of antigens by this technic is simple, they are more stable and easily stored.
Published
1959-01-04
How to Cite
Corrêa, M. O. A., Silva, M. de B. e, & Amato Neto, V. (1959). Ultrasonic waves in the preparation of an antigen for the diagnosis of chagas’ disease. Revista Do Instituto Adolfo Lutz, 19(1-2), 5-8. Retrieved from https://periodicoshomolog.saude.sp.gov.br/index.php/RIAL/article/view/33322
Issue
Vol. 19 No. 1-2 (1959)
Section
ORIGINAL ARTICLE

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